J Clin Med Res
Journal of Clinical Medicine Research, ISSN 1918-3003 print, 1918-3011 online, Open Access
Article copyright, the authors; Journal compilation copyright, J Clin Med Res and Elmer Press Inc
Journal website http://www.jocmr.org

Original Article

Volume 7, Number 8, August 2015, pages 607-612


Factors Predicting Therapeutic Efficacy of Combination Treatment With Sitagliptin and Insulin in Type 2 Diabetic Patients: The ASSIST-K Study

Masashi Ishikawaa, Masahiko Takaia, Hajime Maedaa, Akira Kanamoria, Akira Kubotaa, Hikaru Amemiyaa, Takashi Iizukaa, Kotaro Iemitsua, Tomoyuki Iwasakia, Goro Ueharaa, Shinichi Umezawaa, Mitsuo Obanaa, Hideaki Kaneshigea, Mizuki Kaneshiroa, Takehiro Kawataa, Nobuo Sasaia, Tatsuya Saitoa, Tetsuo Takumaa, Hiroshi Takedaa, Keiji Tanakaa, Nobuaki Tsuruia, Shigeru Nakajimaa, Kazuhiko Hoshinoa, Shin Hondaa, Hideo Machimuraa, Kiyokazu Matobaa, Fuyuki Minagawaa, Nobuaki Minamia, Yukiko Miyairia, Atsuko Mokuboa, Tetsuya Motomiyaa, Manabu Wasedaa, Masaaki Miyakawaa, Yoshikazu Nakaa, Yasuo Terauchib, Yasushi Tanakac, Ikuro Matsubaa, d

aThe Study Group of the Diabetes Committee, Kanagawa Physicians Association, 3-1 Fujimi-cho, Naka-ku, Yokoyama City, Kanagawa 231-0037, Japan
bDepartment of Endocrinology and Metabolism, Yokohama City University, 3-9 Fukuura, Kanazawa-ku, Yokohama 236-0004, Japan
cDivision of Metabolism and Endocrinology, Department of Internal Medicine, St. Marianna University School of Medicine, 2-16-1 Sugao, Miyamae-ku, Kawasaki City, Kanagawa 216-8511, Japan
dCorresponding Author: Ikuro Matsuba, Matsuba Medical Clinic, 2-159 Tsukagoshi, Saiwai-ku, Kawasaki City, Kanagawa 212-0024, Japan

Manuscript accepted for publication March 19, 2015
Short title: Efficacy of Insulin and Sitagliptin
doi: http://dx.doi.org/10.14740/jocmr2149w

Abstract▴Top 

Background: It is unclear whether dipeptidyl peptidase-4 inhibitors decrease hemoglobin A1c (HbA1c) in a glucose-dependent manner in patients on insulin therapy who have impaired insulin secretion. This study investigated factors influencing the efficacy of sitagliptin when used concomitantly with insulin to treat type 2 diabetes mellitus (T2DM) in the real-world setting.

Methods: A retrospective study was conducted of 1,004 T2DM patients at 36 Japanese clinics associated with the Diabetes Task Force of the Kanagawa Physicians Association. Eligible patients had been on insulin for at least 6 months, with a baseline HbA1c of 7.0% (53 mmol/mol) or higher. Baseline characteristics and laboratory data from 495 patients were subjected to multiple regression analysis to identify factors influencing the change of HbA1c.

Results: Most patients (n = 809) received sitagliptin at a dose of 50 mg. In the 1,004 patients, HbA1c decreased by 0.74% (6 mmol/mol) and body weight increased by 0.1 kg after 6 months of combination therapy. Multiple regression analysis showed that a higher baseline HbA1c, older age, and lower body mass index influenced the change of HbA1c after 6 months. Hypoglycemic symptoms occurred in 7.4%, but none were severe.

Conclusions: These results emphasize the importance of a higher HbA1c at the commencement of sitagliptin therapy in patients on insulin. Glucose-dependent suppression of glucagon secretion by sitagliptin may be useful in patients with impaired insulin secretion. Sitagliptin can be used concomitantly with insulin irrespective of the insulin regimen, duration of insulin treatment, and concomitant medications.

Keywords: Type 2 diabetes; Sitagliptin; Insulin; Combination therapy; HbA1c; Multiple regression analysis; Body weight

Introduction▴Top 

Among the dipeptidyl peptidase-4 (DPP-4) inhibitors that are widely used, sitagliptin is the first to be approved for patients with type 2 diabetes mellitus (T2DM) on insulin therapy. Despite its established position, relatively few clinical studies of sitagliptin have been performed. These include a US study of patients who were predominantly on once-daily long-acting, insulin therapy [1], a Japanese study of patients on mixed insulin therapy [2], and observational studies with small populations [3, 4].

T2DM patients receiving insulin often have a longer disease duration and a reduced insulin secretion capacity. Because their baseline characteristics and insulin doses vary, clinical trials alone do not provide enough information about these patients [5]. Moreover, the effectiveness of DPP-4 inhibitors at stimulating an insulin response in patients with compromised insulin secretion on insulin therapy has not been examined. A small-scale randomized controlled trial (RCT) has shown that sitagliptin improves hemoglobin A1c (HbA1c) and postprandial glucose levels in patients with type 1 diabetes [6]. The effect of sitagliptin in increasing glucagon-like peptide 1 (GLP-1), which results in inhibition of glucagon secretion rather than stimulation of insulin secretion, has attracted attention [7, 8].

RCTs play a significant role in evidence-based medicine. However, such trials exclude patients who do not meet the specified inclusion criteria, even though these patients are often important in the real-world clinical setting. This is particularly relevant for T2DM, since pathological conditions vary widely in patients with this disease, making it difficult to investigate all patient types in a single RCT.

In the present study, we investigated the efficacy and safety of insulin-sitagliptin combination therapy in a multicenter study to verify the usefulness of this regimen for T2DM patients on various types of insulin therapy. Our findings add some useful information to the evidence already established by RCTs.

Methods▴Top 

This retrospective study included patients receiving sitagliptin in addition to insulin from November 2011 to March 2013 at 36 diabetes clinics in Kanagawa Prefecture, Japan. Sitagliptin was started if the attending physician considered that insulin was not achieving adequate glycemic control. Glycemic control, the insulin dose, concomitant drugs, blood pressure (BP), body weight, and laboratory data were analyzed, as well as the occurrence of adverse events. Eligible patients had been on insulin for 6 months or longer, with an HbA1c of 7.0% (53 mmol/mol) or higher, and had undergone follow-up for at least 6 months.

The primary endpoint was the change of HbA1c. To explore factors contributing to the improvement of HbA1c by insulin-sitagliptin combination therapy, multiple regression analysis was performed with the following variables: age, sex, baseline body mass index (BMI), duration of insulin therapy, baseline HbA1c, daily insulin dose, presence/absence of diabetic neuropathy, smoking, alcohol consumption, and concomitant use of sulfonylureas (SUs), biguanides (BGs), alpha-glucosidase inhibitors (α-GIs), and thiazolidinediones (TZDs). Patients who had previously been treated with a DPP-4 inhibitor were excluded.

Results are reported as mean ± standard deviation. All analyses were carried out using SPSS version 19 software (SPSS Inc., Chicago, IL, USA). The effect of sitagliptin treatment was assessed by one-way analysis of variance and P < 0.05 was accepted as indicating statistical significance.

This study was registered with the Clinical Trials Registry (http://clinicaltrials.gov; NCT01855087) and was undertaken in accordance with the Ethical Guidelines for Clinical Studies of the Japanese Ministry of Health, Labor, and Welfare. Informed consent was not required because this was a retrospective analysis.

Results▴Top 

A total of 1,169 case record forms were collected. Then patients with sitagliptin treatment for < 6 months (n = 54), baseline HbA1c < 7.0% (53 mmol/mol) (n = 81), and incomplete data (n = 30) were excluded. The remaining 1,004 patients were available for analysis and their characteristics are shown in Table 1. Based on the results of Japanese clinical studies employing sitagliptin, the drug was up-titrated in some patients from a starting dose of 50 mg to 100 mg if the former dose was not effective, while a low dose of 25 mg was selected for 65 patients with impaired renal function [9]. Among the 1,004 patients, medications other than insulin and sitagliptin were altered in 185 patients, including 144 who discontinued α-GIs or TZDs and 41 who received add-on biguanides.

Table 1.
Click to view
Table 1. Characteristics of the Subjects
 

After starting insulin-sitagliptin combination therapy, HbA1c decreased significantly from the baseline value of 8.69±1.31% (72 ± 12 mmol/mol) to 8.30±1.23% (67 ± 11 mmol/mol), 7.99±1.25% (64 ± 11 mmol/mol), and 7.95±1.25% (64 ± 11 mmol/mol) at 1 month, 3 months, and 6 months, respectively. Body weight showed a slight, but significant, increase of 0.1 kg, while there were no changes in the doses of insulin or the other oral antidiabetic drugs (Fig. 1).

Figure 1.
Click for large image
Figure 1. Changes in hemoglobin A1c, body weight, and insulin dose (n = 1,004). Data are the mean ± standard deviation. Analysis of variance vs. baseline. *P < 0.05. M: months.

Hypoglycemic symptoms were noted in 7.4% of the subjects, but were not severe. Other adverse events included abdominal distension (0.9%) and constipation (0.4%). With regard to changes in laboratory values, there was a significant decrease of systolic BP, fasting blood glucose, glycated albumin, alkaline phosphatase, total cholesterol, high-density lipoprotein (HDL) cholesterol, and triglycerides, while there was a significant increase of uric acid and serum creatinine (Table 2).

Table 2.
Click to view
Table 2. Changes in Laboratory Values
 

Multiple regression analysis was performed to examine factors related to the change of HbA1c within 6 months of starting sitagliptin. Complete baseline data on the age, sex, BMI, duration of insulin treatment, HbA1c, daily insulin dose, presence/absence of diabetic neuropathy, smoking, alcohol consumption, and concomitant use of SUs, BGs, α-GIs and TZDs were available for 495 patients. These characteristics of the 495 patients were similar to those of the main study population (data not shown).

A high HbA1c at baseline was the strongest contributor to reduction of HbA1c at 6 months after starting treatment with insulin-sitagliptin combination therapy (Table 3). In addition, BMI and age made slight, but significant, contributions to the reduction of HbA1c (Table 3).

Table 3.
Click to view
Table 3. Multiple Regression Analysis of Delta Hemoglobin A1c After 6 Months of Insulin-Sitagliptin Combination Therapy in 495 Patients With Complete Data
 

We also performed multiple regression analysis on a cohort of 114 patients who had C-peptide data before commencement of sitagliptin therapy. This showed that C-peptide had no influence on the effectiveness of sitagliptin (data not shown).

Discussion▴Top 

The Diabetes Task Force of the Kanagawa Physicians Association previously conducted a large-scale trial involving more than 1,000 patients (the ASSET-K study), which established that a higher baseline HbA1c, shorter disease duration, and lower baseline BMI were factors contributing to reduction of HbA1c in patients receiving sitagliptin combined with another oral antidiabetic drug [10-14]. However, the factors influencing the HbA1c-lowering effect of sitagliptin when it is used concomitantly with insulin were not fully elucidated. Therefore, we conducted the present study to investigate this issue.

We confirmed the effectiveness and safety of combining sitagliptin with insulin in a large patient population (n = 1,004) treated in the real-world setting. HbA1c decreased by 0.74% (6 mmol/mol) and body weight increased by 0.1 kg, while there were no appreciable changes in the doses of insulin or other oral antidiabetic agents. In addition, there were no episodes of severe hypoglycemia, although hypoglycemic events were reported in 7.4% of the patients. In general, the formulation, dose, and frequency of insulin administration are altered if an HbA1c < 7.0% (53 mmol/mol) is not achieved after a certain period. However, increasing the insulin dose may lead to adverse events such as weight gain and hypoglycemia. The present study demonstrated the effectiveness of insulin-sitagliptin combination therapy in the real-world setting. As well as improving HbA1c, there were significant reductions of systolic BP, fasting blood glucose, glycated albumin, alkaline phosphatase, total cholesterol, HDL cholesterol, and triglycerides, which suggest that sitagliptin may also exert a favorable effect on BP and the lipid profile, as reported in the ASSET-K study [14]. The significant increase of uric acid and serum creatinine were also in agreement with the results of the ASSET-K study, and can be explained by the fact that sitagliptin increases GLP-1, an incretin hormone with diuretic properties. In fact, a correlation between the reduction of BP and an increase of creatinine and uric acid levels has been reported [14].

We performed multiple regression analysis to identify patients who were likely to respond to addition of sitagliptin to insulin therapy. We analyzed various factors that had a potential influence on HbA1c including diabetic neuropathy, smoking, and alcohol, because diabetic neuropathy may inhibit the effect of GLP-1 and patients who smoke or drink regularly are more likely to lead an unhealthy lifestyle with suboptimal diet and exercise. We identified a higher baseline HbA1c as the strongest contributing factor, while a lower baseline BMI and older age were also significant. This is in agreement with the fact that older patients tended to have a lower BMI in our study. There was a positive correlation between the duration of diabetes and the duration of insulin therapy (Pearson’s correlation coefficient = 0.40), as well as between the frequency of insulin administration and the daily insulin dose (Pearson’s correlation coefficient = 0.51). Therefore, the duration of insulin therapy and the daily insulin dose were included in the multiple regression analysis as background factors, revealing that the duration of insulin therapy was not associated with the effectiveness of sitagliptin. These results support the addition of sitagliptin to insulin therapy, irrespective of the duration of insulin treatment, the daily insulin dose, concomitant medications, and the C-peptide profile. Even in patients with compromised insulin secretion, sitagliptin can still effectively stimulate insulin secretion and it also has other potential benefits. Inhibition of glucagon secretion may be involved in the efficacy of DPP-4 inhibitors for insulin-treated patients [6] and inhibition of glucagon secretion as a GLP-1 receptor agonist may be important in type 1 diabetes [15].

It has been reported that T2DM patients in East Asian countries (including Japan) have lower endogenous insulin secretion and that their BMI tends to be lower [16]. Kim et al reported that sitagliptin was particularly effective in the Japanese population [17]. In addition to the ASSET-K study, several observational studies have demonstrated the effectiveness of sitagliptin in patients with a lower BMI [11, 13, 18]. However, more research is needed to determine whether our results are related to ethnic factors, especially studies in non-Japanese populations.

There were some limitations of this study. First, it was retrospective. Second, all of the patients received sitagliptin and there was no control group without sitagliptin treatment. Third, markers for the assessment of beta-cell function (HOMA-beta) and the basal level of active GLP-1, which are factors that could influence the reduction of HbA1c by sitagliptin therapy, were not measured in all patients. In fact, only 495 out of 1,004 patients were included in the detailed analysis, but these patients were considered to be reasonably representative of the entire population since their characteristics did not differ from those of the whole population (data not shown).

In conclusion, this study provided useful new information about the efficacy and safety of sitagliptin that can be added to the findings of relevant RCTs. We demonstrated that a higher baseline HbA1c was associated with greater improvement of HbA1c in patients given sitagliptin as add-on therapy to insulin, while other factors were not clinically relevant. This suggests that patients might be candidates for add-on sitagliptin therapy irrespective of their characteristics, insulin dose, and concomitant medications. Because a significant increase of body weight was not observed, sitagliptin may be added whenever the HbA1c is not well controlled by insulin therapy.

Acknowledgement

The authors would like to thank the members of the Kanagawa Physicians Association.

Competing Interests

MI, MT, and IM have received lecture fees from MSD K.K. and Ono Pharmaceutical, Co., Ltd.

Grant Support

This research was financially supported by the Kidney Foundation, Japan.


References▴Top 
  1. Umpierrez GE, Gianchandani R, Smiley D, Jacobs S, Wesorick DH, Newton C, Farrokhi F, et al. Safety and efficacy of sitagliptin therapy for the inpatient management of general medicine and surgery patients with type 2 diabetes: a pilot, randomized, controlled study. Diabetes Care. 2013;36(11):3430-3435.
    doi pubmed
  2. Katsuno T, Ikeda H, Ida K, Miyagawa J, Namba M. Add-on therapy with the DPP-4 inhibitor sitagliptin improves glycemic control in insulin-treated Japanese patients with type 2 diabetes mellitus. Endocr J. 2013;60(6):733-742.
    doi pubmed
  3. Shimoda S, Iwashita S, Ichimori S, Matsuo Y, Goto R, Maeda T, Matsuo T, et al. Efficacy and safety of sitagliptin as add-on therapy on glycemic control and blood glucose fluctuation in Japanese type 2 diabetes subjects ongoing with multiple daily insulin injections therapy. Endocr J. 2013;60(10):1207-1214.
    doi pubmed
  4. Mori Y, Taniguchi Y, Miyazaki S, Yokoyama J, Utsunomiya K. Effects of add-on treatment with sitagliptin on narrowing the range of glucose fluctuations in Japanese type 2 diabetes patients receiving insulin therapy. Diabetes Technol Ther. 2013;15(3):237-240.
    doi pubmed
  5. Kadowaki T, Tajima N, Odawara M, Minamide T, Kawashima M, Yanagida D, Okamoto T, et al. Efficacy and safety of sitagliptin add-on therapy in Japanese patients with type 2 diabetes on insulin monotherapy. Diabetol Int. 2013;4:160-172.
    doi
  6. Ellis SL, Moser EG, Snell-Bergeon JK, Rodionova AS, Hazenfield RM, Garg SK. Effect of sitagliptin on glucose control in adult patients with Type 1 diabetes: a pilot, double-blind, randomized, crossover trial. Diabet Med. 2011;28(10):1176-1181.
    doi pubmed
  7. Watanabe K, Kobayashi K, Takemoto M, Ishibashi R, Yamaga M, Kawamura H, Fujimoto M, et al. Sitagliptin improves postprandial hyperglycemia by inhibiting glucagon secretion in Werner syndrome with diabetes. Diabetes Care. 2013;36(8):e119.
    doi pubmed
  8. Chen B, Moore A, Escobedo LV, Koletsky MS, Hou D, Koletsky RJ, Ernsberger P. Sitagliptin lowers glucagon and improves glucose tolerance in prediabetic obese SHROB rats. Exp Biol Med (Maywood). 2011;236(3):309-314.
    doi pubmed
  9. Iwamoto Y, Taniguchi T, Nonaka K, Okamoto T, Okuyama K, Arjona Ferreira JC, Amatruda J. Dose-ranging efficacy of sitagliptin, a dipeptidyl peptidase-4 inhibitor, in Japanese patients with type 2 diabetes mellitus. Endocr J. 2010;57(5):383-394.
    doi pubmed
  10. Maeda H, Kubota A, Tanaka Y, Terauchi Y, Matsuba I. The safety, efficacy and predictors for HbA1c reduction of sitagliptin in the treatment of Japanese type 2 diabetes. Diabetes Res Clin Pract. 2012;95(1):e20-22.
    doi pubmed
  11. Maeda H, Kubota A, Kanamori A, Tanaka Y, Terauchi Y, Matsuba I. Long-term efficacy and safety of sitagliptin in the treatment of Japanese Type 2 diabetes (ASSET-K1) to a target of HbA1c <7%. J Endocrinol Invest. 2013;36(8):568-573.
    pubmed
  12. Kanamori A, Matsuba I. Factors associated with reduced efficacy of sitagliptin therapy: analysis of 93 patients with type 2 diabetes treated for 1.5 years or longer. J Clin Med Res. 2013;5(3):217-221.
    doi
  13. Kubota A, Maeda H, Kanamori A, Matoba K, Jin Y, Minagawa F, Obana M, et al. Efficacy and safety of sitagliptin monotherapy and combination therapy in Japanese type 2 diabetes patients. J Diabetes Investig. 2012;3(6):503-509.
    doi pubmed
  14. Kubota A, Maeda H, Kanamori A, Matoba K, Jin Y, Minagawa F, Obana M, et al. Pleiotropic effects of sitagliptin in the treatment of type 2 diabetes mellitus patients. J Clin Med Res. 2012;4(5):309-313.
    doi
  15. Kielgast U, Krarup T, Holst JJ, Madsbad S. Four weeks of treatment with liraglutide reduces insulin dose without loss of glycemic control in type 1 diabetic patients with and without residual beta-cell function. Diabetes Care. 2011;34(7):1463-1468.
    doi pubmed
  16. Kim YG, Hahn S, Oh TJ, Kwak SH, Park KS, Cho YM. Differences in the glucose-lowering efficacy of dipeptidyl peptidase-4 inhibitors between Asians and non-Asians: a systematic review and meta-analysis. Diabetologia. 2013;56(4):696-708.
    doi pubmed
  17. Park H, Park C, Kim Y, Rascati KL. Efficacy and safety of dipeptidyl peptidase-4 inhibitors in type 2 diabetes: meta-analysis. Ann Pharmacother. 2012;46(11):1453-1469.
    doi pubmed
  18. Kim SA, Shim WH, Lee EH, Lee YM, Beom SH, Kim ES, Yoo JS, et al. Predictive clinical parameters for the therapeutic efficacy of sitagliptin in korean type 2 diabetes mellitus. Diabetes Metab J. 2011;35(2):159-165.
    doi pubmed


This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.


Journal of Clinical Medicine Research is published by Elmer Press Inc.



 

 

 

 

Browse  Journals  

     

Journal of clinical Medicine Research

Journal of Endocrinology and Metabolism

Journal of Clinical Gynecology and Obstetrics

World Journal of Oncology

Gastroenterology Research

Journal of Hematology

Journal of Medical Cases

Journal of Current Surgery

Clinical Infection and Immunity

Cardiology Research

World Journal of Nephrology and Urology

Cellular and Molecular Medicine Research

Journal of Neurology Research

International Journal of Clinical Pediatrics

 

 

 

 

 

Journal of Clinical Medicine Research, monthly, ISSN 1918-3003 (print), 1918-3011 (online), published by Elmer Press Inc.            
The content of this site is intended for health care professionals.
This is an open-access journal distributed under the terms of the Creative Commons Attribution-NonCommercial 4.0 International License, which permits unrestricted
non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.
Creative Commons Attribution license (Attribution-NonCommercial 4.0 International CC-BY-NC 4.0)


This journal follows the International Committee of Medical Journal Editors (ICMJE) recommendations for manuscripts submitted to biomedical journals,
the Committee on Publication Ethics (COPE) guidelines, and the Principles of Transparency and Best Practice in Scholarly Publishing.

website: www.jocmr.org   editorial contact: editor@jocmr.org
Address: 9225 Leslie Street, Suite 201, Richmond Hill, Ontario, L4B 3H6, Canada

© Elmer Press Inc. All Rights Reserved.