J Clin Med Res

Journal of Clinical Medicine Research, ISSN 1918-3003 print, 1918-3011 online, Open Access

Article copyright, the authors; Journal compilation copyright, J Clin Med Res and Elmer Press Inc

Journal website http://www.jocmr.org

Original Article

Volume 10, Number 3, March 2018, pages 189-195

Association of SIRT-1 Gene Polymorphism and Vitamin D Level in Egyptian Patients With Rheumatoid Arthritis

**Table 1.** Demographic Characteristics and Routine Investigations in Different Study Groups
Variables	Rheumatoid cases (n = 50), mean ± SD	Controls (n = 20), mean ± SD	P-value
Independent t-test: Pearson χ² (exact). SD: standard deviation.
Sex
Males	38 (76%)	9 (45%)	0.023
Females	12 (24%)	11 (55%)
Age (years)	40.1 ± 11.5	43.3 ± 9.8	0.289
Complete blood count
RBCs (g/dL)	92.4 ± 26.6	100.7 ± 16.5	0.199
Hb (g/dL)	13.4 ± 1.9	13.8 ± 1.7	0.429
TLC (10/mm)	6.6 ± 2	5.7 ± 0.98	0.047
PLT (10/mm)	272.9 ± 94.7	239.1 ± 68.6	0.150
Liver function
ALT (IU/L)	44.6 ± 13.6	32.5 ± 5.7	0.000
AST (IU/L)	52.6 ± 10.1	30.9 ± 5.5	0.000
Total bilirubin (mg/dL)	0.72 ± 0.17	0.85 ± 0.23	0.036
Direct bilirubin (mg/dL)	0.22 ± 0.10	0.23 ± 0.11	0.851
Albumin (g/dL)	4.1 ± 0.48	4.3 ± 0.41	0.058
Other investigations
Alkaline phosphatase (IU/L)	67.2 ± 26	71.4 ± 23	0.536
Creatinine (mg/dL)	0.91 ± 0.29	0.95 ± 0.18	0.02
CRP (mg/L)	0.24 ± 0.13	0.23 ± 0.09	0.707
INR	1.07 ± 0.10	1.12 ± 0.13	0.143
Anti-CCP (U/ML)	61.7 ± 26	6.9 ± 4.5	0.000
TSH (mIU/L)	1.7 ± 0.99	1.7 ± 0.77	0.799
AFP (ng/mL)	2.9 ± 2.1	1.7 ± 0	0.012
Vit D (ng/mL)	17.9 ± 9.2	42.2 ± 11.8	0.000

Table 1. Demographic Characteristics and Routine Investigations in Different Study Groups

Variables

Rheumatoid cases (n = 50), mean ± SD

Controls (n = 20), mean ± SD

P-value

Independent t-test: Pearson χ² (exact). SD: standard deviation.

Sex

Males

38 (76%)

9 (45%)

0.023

Females

12 (24%)

11 (55%)

Age (years)

40.1 ± 11.5

43.3 ± 9.8

0.289

Complete blood count

RBCs (g/dL)

92.4 ± 26.6

100.7 ± 16.5

0.199

Hb (g/dL)

13.4 ± 1.9

13.8 ± 1.7

0.429

TLC (10/mm)

6.6 ± 2

5.7 ± 0.98

0.047

PLT (10/mm)

272.9 ± 94.7

239.1 ± 68.6

0.150

Liver function

ALT (IU/L)

44.6 ± 13.6

32.5 ± 5.7

0.000

AST (IU/L)

52.6 ± 10.1

30.9 ± 5.5

0.000

Total bilirubin (mg/dL)

0.72 ± 0.17

0.85 ± 0.23

0.036

Direct bilirubin (mg/dL)

0.22 ± 0.10

0.23 ± 0.11

0.851

Albumin (g/dL)

4.1 ± 0.48

4.3 ± 0.41

0.058

Other investigations

Alkaline phosphatase (IU/L)

67.2 ± 26

71.4 ± 23

0.536

Creatinine (mg/dL)

0.91 ± 0.29

0.95 ± 0.18

0.02

CRP (mg/L)

0.24 ± 0.13

0.23 ± 0.09

0.707

INR

1.07 ± 0.10

1.12 ± 0.13

0.143

Anti-CCP (U/ML)

61.7 ± 26

6.9 ± 4.5

0.000

TSH (mIU/L)

1.7 ± 0.99

1.7 ± 0.77

0.799

AFP (ng/mL)

2.9 ± 2.1

1.7 ± 0

0.012

Vit D (ng/mL)

17.9 ± 9.2

42.2 ± 11.8

0.000

**Table 2.** ANA Titer and Vitamin D Levels in Study Groups
Variables	Rheumatoid cases (n = 50)	Controls (n = 20)	P-value
ANA titer
Low titer	50	100%	20	100%	-
Vitamin D level					0.000
Deficient (< 10 ng/mL)	3	6%	0	0%
Insufficient (10 - 29 ng/mL)	44	88%	0	0%
Sufficient (30 - 100 ng/mL)	3	6%	20	100%

Table 2. ANA Titer and Vitamin D Levels in Study Groups

Variables

Rheumatoid cases (n = 50)

Controls (n = 20)

P-value

No.

ANA titer

Low titer

100%

Vitamin D level

0.000

Deficient (< 10 ng/mL)

Insufficient (10 - 29 ng/mL)

88%

Sufficient (30 - 100 ng/mL)

100%

**Table 3.** Distribution of Genotypes and Allelic Frequency Among Patients and Controls
Variables	Rheumatoid cases (n = 50)	Controls (n = 20)	P-value
rs2273773 C>T
CC	18	36%	12	60%
CT	19	38%	8	40%	0.000
TT	13	26%	0	0%
Allele
C	55	55%	32	80%	0.006
T	45	45%	8	20%
rs7895833 A>G
GG	27	54%	2	10%
AG	17	34%	6	30%	0.000
AA	6	12%	12	60%
Allele
G	71	71%	10	25%	< 0.001
A	29	29%	30	75%
rs7069102 C>G
GG	31	62%	0	0%
CG	13	26%	5	25%	0.000
CC	6	12%	15	75%
Allele
C	25	25%	35	87.5%	< 0.001
G	75	75%	5	12.5%

Table 3. Distribution of Genotypes and Allelic Frequency Among Patients and Controls

Variables

Rheumatoid cases (n = 50)

Controls (n = 20)

P-value

No.

rs2273773 C>T

36%

60%

38%

40%

0.000

26%

Allele

55%

80%

0.006

45%

20%

rs7895833 A>G

54%

10%

34%

30%

0.000

12%

60%

Allele

71%

25%

< 0.001

29%

75%

rs7069102 C>G

62%

26%

25%

0.000

12%

75%

Allele

25%

87.5%

< 0.001

75%

12.5%

**Table 4.** Comparisons of Vitamin D in Different SIRT-1 Polymorphisms Among Rheumatoid Patients
Variables	Vitamin D	P-value
rs2273773
TT	17.3	9.5
TC	16.3	4.7	0.4
CC	20.9	13.1
rs7895833
GG	17.4	6.1
AG	19.9	13.5	0.4
AA	13.9	4.6
rs7069102
GG	15.9	4.5
CG	22.1	15.7	0.2
CC	16.5	5.2

Table 4. Comparisons of Vitamin D in Different SIRT-1 Polymorphisms Among Rheumatoid Patients

Variables

Vitamin D

P-value

Mean

rs2273773

17.3

9.5

16.3

4.7

0.4

20.9

13.1

rs7895833

17.4

6.1

19.9

13.5

0.4

13.9

4.6

rs7069102

15.9

4.5

22.1

15.7

0.2

16.5

5.2

**Table 5.** Comparisons of Anti-CCP in Different SIRT-1 Polymorphisms Among Rheumatoid Patients
Variables	Anti-CCP	P-value
rs2273773
TT	59.6	23.4
TC	59.1	17.4	0.6
CC	68.5	38.3
rs7895833
GG	67.9	29.9
AG	58.9	18.6	0.07
AA	41.8	13
rs7069102
GG	58.2	23.1
CG	71.7	39	0.3
CC	58.2	18.7

Table 5. Comparisons of Anti-CCP in Different SIRT-1 Polymorphisms Among Rheumatoid Patients

Variables

Anti-CCP

P-value

Mean

rs2273773

59.6

23.4

59.1

17.4

0.6

68.5

38.3

rs7895833

67.9

29.9

58.9

18.6

0.07

41.8

rs7069102

58.2

23.1

71.7

0.3

58.2

18.7

**Table 6.** Correlation Between Vitamin D Levels With All Study Variables
Variables	Vitamin D
Age (years)	0.19	0.2
Complete blood count
RBCs	0.15	0.3
Hb	-0.11	0.5
TLC	0.09	0.5
PLT	0.02	0.9
Liver function
ALT	-0.16	0.3
AST	0.04	0.8
Total bilirubin	0.27	0.09
Direct bilirubin	0.25	0.08
Albumin	-0.26	0.07
Other routine investigations
Alkaline phosphatase	0.07	0.6
Creatinine	-0.19	0.2
PC	0.04	0.8
CRP	-0.06	0.6
INR	0.14	0.3
Other
Anti-CCP	0.14	0.4
TSH	-0.16	0.3
AFP	-0.19	0.2

Table 6. Correlation Between Vitamin D Levels With All Study Variables

Variables

Vitamin D

P-value

Age (years)

0.19

0.2

Complete blood count

RBCs

0.15

0.3

-0.11

0.5

TLC

0.09

0.5

PLT

0.02

0.9

Liver function

ALT

-0.16

0.3

AST

0.04

0.8

Total bilirubin

0.27

0.09

Direct bilirubin

0.25

0.08

Albumin

-0.26

0.07

Other routine investigations

Alkaline phosphatase

0.07

0.6

Creatinine

-0.19

0.2

0.04

0.8

CRP

-0.06

0.6

INR

0.14

0.3

Other

Anti-CCP

0.14

0.4

TSH

-0.16

0.3

AFP

-0.19

0.2